Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA
Identifieur interne : 005887 ( Main/Exploration ); précédent : 005886; suivant : 005888Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA
Auteurs : Li-Rung Huang [Taïwan] ; Chi-Ming Chiu [Taïwan] ; Shiou-Hwei Yeh [Taïwan] ; Wen-Hung Huang [Taïwan] ; Po-Ren Hsueh [Taïwan] ; Wen-Zieh Yang [Taïwan] ; Jyh-Yuan Yang [Taïwan] ; Ih-Jen Su [Taïwan] ; Shan-Chwen Chang [Taïwan] ; Pei-Jer Chen [Taïwan]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2004-07.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (biosynthèse), Anticorps antiviraux (sang), Antigènes viraux (génétique), Antigènes viraux (immunologie), Clonage moléculaire, Données de séquences moléculaires, Escherichia coli (génétique), Escherichia coli (métabolisme), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Production d'anticorps, Protéines de fusion recombinantes (immunologie), Protéines de fusion recombinantes (isolement et purification), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Séquence d'acides aminés, Technique de Western, Test ELISA, Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Anticorps antiviraux.
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Antigènes viraux, Escherichia coli, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines nucléocapside.
- immunologie : Antigènes viraux, Glycoprotéines membranaires, Protéines de fusion recombinantes, Protéines de l'enveloppe virale, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Protéines de fusion recombinantes.
- métabolisme : Escherichia coli.
- sang : Anticorps antiviraux.
- Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Production d'anticorps, Séquence d'acides aminés, Technique de Western, Test ELISA.
- Pascal (Inist)
English descriptors
- KwdEn :
- Amino Acid Sequence, Antibodies, Viral (biosynthesis), Antibodies, Viral (blood), Antibody, Antibody Formation, Antigens, Viral (genetics), Antigens, Viral (immunology), Blotting, Western, Cloning, Molecular, ELISA assay, Enzyme-Linked Immunosorbent Assay, Escherichia coli (genetics), Escherichia coli (metabolism), Humans, Immunoblotting assay, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Molecular Sequence Data, Nucleocapsid, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Protein, Recombinant Fusion Proteins (immunology), Recombinant Fusion Proteins (isolation & purification), SARS Virus (immunology), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Vitronectin.
- MESH :
- chemical , biosynthesis : Antibodies, Viral.
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Antigens, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins.
- chemical , immunology : Antigens, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Recombinant Fusion Proteins, Viral Envelope Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : Escherichia coli.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- chemical , isolation & purification : Recombinant Fusion Proteins.
- metabolism : Escherichia coli.
- Amino Acid Sequence, Antibody Formation, Blotting, Western, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay, Humans, Molecular Sequence Data, Spike Glycoprotein, Coronavirus.
Abstract
Severe acute respiratory syndrome (SARS)‐CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. To study the humoral responses against SARS‐CoV, we evaluated nucleocapsid (N) and spike (S) proteins‐specific antibodies in patients' sera by Western blotting and enzyme‐linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS‐CoV were purified from transformed E. coli. Serum specimens from 40 SARS‐CoV‐infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT‐PCR‐confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein‐specific antibodies whereas only 47.5% of the samples were positive for S protein‐specific antibodies. Using N protein‐based ELISA, 10 out of the 12 patients were positive for N protein‐specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS‐CoV infection. Antibodies against SARS‐CoV N protein appear at the early stage of infection, therefore, N protein‐based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS‐CoV infection. J. Med. Virol. 73:338–346, 2004. © 2004 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.20096
Affiliations:
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first="Li-Rung" last="Huang">Li-Rung Huang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chiu, Chi Ing" sort="Chiu, Chi Ing" uniqKey="Chiu C" first="Chi-Ming" last="Chiu">Chi-Ming Chiu</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yeh, Shiou Wei" sort="Yeh, Shiou Wei" uniqKey="Yeh S" first="Shiou-Hwei" last="Yeh">Shiou-Hwei Yeh</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Division of Molecular and Genomic Medicine, National Health Research Institutes, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Wen Ung" sort="Huang, Wen Ung" uniqKey="Huang W" first="Wen-Hung" last="Huang">Wen-Hung Huang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Hsueh, Po En" sort="Hsueh, Po En" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Internal Medicine, National Taiwan University Hospital, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Wen Ieh" sort="Yang, Wen Ieh" uniqKey="Yang W" first="Wen-Zieh" last="Yang">Wen-Zieh Yang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Center for Disease Control, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Jyh Uan" sort="Yang, Jyh Uan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Center for Disease Control, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Su, Ih En" sort="Su, Ih En" uniqKey="Su I" first="Ih-Jen" last="Su">Ih-Jen Su</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Center for Disease Control, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chang, Shan Hwen" sort="Chang, Shan Hwen" uniqKey="Chang S" first="Shan-Chwen" last="Chang">Shan-Chwen Chang</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Internal Medicine, National Taiwan University Hospital, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Pei Er" sort="Chen, Pei Er" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Graduate Institute of Microbiology, National Taiwan University College of Medicine, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Internal Medicine, National Taiwan University Hospital, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Taïwan</country></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Taïwan</country><wicri:regionArea>Correspondence address: Director, Graduate Institute of Clinical Medicine, National Taiwan University College of Medicine, 1 Chang‐Te Street, Taipei 100</wicri:regionArea><wicri:noRegion>Taipei 100</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">73</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="338">338</biblScope><biblScope unit="page" to="346">346</biblScope><biblScope unit="page-count">9</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2004-07">2004-07</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibodies, Viral (blood)</term><term>Antibody</term><term>Antibody Formation</term><term>Antigens, Viral (genetics)</term><term>Antigens, Viral (immunology)</term><term>Blotting, Western</term><term>Cloning, Molecular</term><term>ELISA assay</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Humans</term><term>Immunoblotting assay</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Molecular Sequence Data</term><term>Nucleocapsid</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Protein</term><term>Recombinant Fusion Proteins (immunology)</term><term>Recombinant Fusion Proteins (isolation & purification)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe acute respiratory syndrome</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term><term>Vitronectin</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (biosynthèse)</term><term>Anticorps antiviraux (sang)</term><term>Antigènes viraux (génétique)</term><term>Antigènes viraux (immunologie)</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Production d'anticorps</term><term>Protéines de fusion recombinantes (immunologie)</term><term>Protéines de fusion recombinantes (isolement et purification)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Séquence d'acides aminés</term><term>Technique de Western</term><term>Test ELISA</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Recombinant Fusion Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term><term>Escherichia coli</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term><term>Glycoprotéines membranaires</term><term>Protéines de fusion recombinantes</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines de fusion recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Antibody Formation</term><term>Blotting, Western</term><term>Cloning, Molecular</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Molecular Sequence Data</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Production d'anticorps</term><term>Séquence d'acides aminés</term><term>Technique de Western</term><term>Test ELISA</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Anticorps</term><term>Méthode immunoblotting</term><term>Nucléocapside</term><term>Protéine</term><term>Syndrome respiratoire aigu sévère</term><term>Technique ELISA</term><term>Vitronectine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS)‐CoV is a newly emerging virus that causes SARS with high mortality rate in infected people. To study the humoral responses against SARS‐CoV, we evaluated nucleocapsid (N) and spike (S) proteins‐specific antibodies in patients' sera by Western blotting and enzyme‐linked immunosorbent assay (ELISA). Recombinant N and S proteins of SARS‐CoV were purified from transformed E. coli. Serum specimens from 40 SARS‐CoV‐infected patients in the convalescent phase were analyzed by Western blotting using the purified antigens. Serial serum specimens from 12 RT‐PCR‐confirmed SARS patients were assayed by ELISA using the recombinant N protein as coated antigen. By Western blotting, 97.5% of the SARS patients were positive for N protein‐specific antibodies whereas only 47.5% of the samples were positive for S protein‐specific antibodies. Using N protein‐based ELISA, 10 out of the 12 patients were positive for N protein‐specific antibodies and 6 of them showed seroconversion at mean of 16 days after onset of fever. Immunoblotting was useful for detecting the humoral immune response after SARS‐CoV infection. Antibodies against SARS‐CoV N protein appear at the early stage of infection, therefore, N protein‐based ELISA could serve as a simple, sensitive, and specific test for diagnosing SARS‐CoV infection. J. Med. Virol. 73:338–346, 2004. © 2004 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Huang, Li Ung" sort="Huang, Li Ung" uniqKey="Huang L" first="Li-Rung" last="Huang">Li-Rung Huang</name></noRegion><name sortKey="Chang, Shan Hwen" sort="Chang, Shan Hwen" uniqKey="Chang S" first="Shan-Chwen" last="Chang">Shan-Chwen Chang</name><name sortKey="Chen, Pei Er" sort="Chen, Pei Er" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><name sortKey="Chen, Pei Er" sort="Chen, Pei Er" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><name sortKey="Chen, Pei Er" sort="Chen, Pei Er" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><name sortKey="Chen, Pei Er" sort="Chen, Pei Er" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><name sortKey="Chen, Pei Er" sort="Chen, Pei Er" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><name sortKey="Chiu, Chi Ing" sort="Chiu, Chi Ing" uniqKey="Chiu C" first="Chi-Ming" last="Chiu">Chi-Ming Chiu</name><name sortKey="Hsueh, Po En" sort="Hsueh, Po En" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name><name sortKey="Huang, Wen Ung" sort="Huang, Wen Ung" uniqKey="Huang W" first="Wen-Hung" last="Huang">Wen-Hung Huang</name><name sortKey="Su, Ih En" sort="Su, Ih En" uniqKey="Su I" first="Ih-Jen" last="Su">Ih-Jen Su</name><name sortKey="Yang, Jyh Uan" sort="Yang, Jyh Uan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name><name sortKey="Yang, Wen Ieh" sort="Yang, Wen Ieh" uniqKey="Yang W" first="Wen-Zieh" last="Yang">Wen-Zieh Yang</name><name sortKey="Yeh, Shiou Wei" sort="Yeh, Shiou Wei" uniqKey="Yeh S" first="Shiou-Hwei" last="Yeh">Shiou-Hwei Yeh</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Evaluation of antibody responses against SARS coronaviral nucleocapsid or spike proteins by immunoblotting or ELISA
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